Protein Dynamics and Time Resolved Protein Crystallography at Synchrotron Radiation Sources: Past, Present and Future

The ultrabright and ultrashort pulses produced at X-ray free electron lasers (XFELs) has enabled studies of crystallized molecular machines work under ‘native’ conditions room temperature by the so-called time-resolved serial femtosecond crystallography (TR-SFX) technique. Since early TR-SFX experiments were conducted XFELs, it been largely reported in literature that synchrotrons are no longer feasible or impractical due to severe technical limitations these radiation sources. transfer approach newest upgraded for higher flux density with beamlines using sophisticated focusing optics, submicron beam diameters fast low-noise photon-counting detectors offers a way overcome difficulties opening new exciting possibilities. In fact, there is an increasing amount publications reporting findings structural dynamics protein macromolecules time resolved from microcrystals synchrotron This review gathers information provide overview recent advances made this filed past years, as well outlines future perspectives next generation sources upcoming compact pulsed